Open AccessOverexpression of eukaryotic translation initiation factor 4E ‐binding protein 1 induces the alteration of immune status in H1299 lung cancer cells
Author(s) -
Li Lei,
Zhang Li,
Liu Dan,
Cheng Yue,
Jing Yuting,
Yu He,
Zhou Ping,
Song Juan,
Li Weimin
Publication year - 2015
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12196
Subject(s) - cell cycle , downregulation and upregulation , microbiology and biotechnology , eif4ebp1 , tensin , initiation factor , eukaryotic initiation factor , immune system , medicine , cancer research , eukaryotic translation , western blot , biology , immunology , messenger rna , cancer , signal transduction , translation (biology) , gene , pten , genetics , pi3k/akt/mtor pathway
Background Eukaryotic translation initiation factor 4E ‐binding protein 1 ( 4E‐BP1 ) is an important factor regulating protein translation. It also impacts proliferation, apoptosis, invasion, and the cell cycle of cancer cells. The aim of this study was to investigate the relationship between 4E‐BP1 and human immune status, recognizing immunomodulatory molecules involved in the overexpression of 4E‐BP1 . Methods A lentivirus expression system was used to overexpress 4E‐BP1 in the H1299 cell line. Western blot was performed to investigate the expression level of 4E‐BP1 and P ‐ 4E‐BP1 , and quantitative polymerase chain reaction was used to quantify gene expression of immunomodulatory molecules. Results The expression level of 4E‐BP1 increased significantly after lentivirus infection ( P < 0.05). Overexpression of 4E‐BP1 upregulated the expression of interleukin ( IL) ‐1β ( P < 0.05), IL ‐5 ( P < 0.001), IL ‐23 ( P < 0.001), macrophage inflammatory protein‐1β ( P < 0.001), Eota‐3 ( P < 0.05), and MCP ‐4 ( P < 0.05). Most of the increases were observed at the seventh day. The variation trend of IL ‐10, cell division cycle protein 2, proliferating cell nuclear antigen, and phosphatase and tensin homolog was not clear. Conclusion Overexpression of 4E‐BP1 altered immune status by upregulating the expression of a series of immunomodulatory molecules, indicating that 4E‐BP1 could serve as a potential therapeutic target against cancer.