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Background  To determine the relationship between  FOXN1  (a transcription factor) and  B  cell‐attracting chemokine 1 ( BCA1 , a chemotactic factor), and their influence on thymoma cell proliferation.    Methods  We initially used immunohistochemical methods to compare the expression levels of  FOXN1  and  BCA1  in thymoma and non‐thymomatous tissue samples. Reverse transcription polymerase chain reaction ( RT‐PCR ) and  W estern blotting were used to compare the expression of  FOXN1  and  BCA1  in thymoma cells ( T hy0517) and normal thymic epithelial cells ( CRL 7660). We used ribonucleic acid interference ( RNAi ) to downregulate  FOXN1  and  BCA1  expression in  T hy0517 cells to determine the relationship of the two factors with cell regulation. We also performed methyl thiazolyl tetrazolium ( MTT)  [3‐(4,5)‐dimethylthiahiazo(‐z‐y1)‐3,5‐di‐phenytetrazoliumromide] assays to detect the changes in  T hy0517 cells after  RNAi  of  FOXN1  and  BCA1 .    Results   FOXN1  and  BCA1  expression levels were higher in thymoma tissues and  T hy0517 cells compared to non‐thymomatous tissue and  CRL 7660 cells ( P  < 0.05).  RT‐PCR  and  W estern blot following  RNAi  showed that  FOXN1  controlled  BCA1  expression.  MTT  assay showed that  FOXN1  and  BCA1  downregulation rapidly inhibited  T hy0517 cell proliferation.    Conclusions   FOXN1  and  BCA1  expression was higher in thymoma tissue samples and cell lines than in non‐thymomatous tissue and normal thymic epithelial cells.  FOXN1  acts upstream of  BCA1  and both  FOXN1  and  BCA1  promote thymoma cell proliferation.

thoracic cancer

In vitro  study of the effect of small interfering ribonucleic acid on the expression of  FOXN1  and  B  cell‐attracting chemokine 1 in thymoma cell lines