Open AccessAnalysis of differently expressed proteins involved in metastatic niche of lung
Author(s) -
Zheng Yan,
Lin Yongbin,
Situ Dongrong,
Jiang Long,
Su Xiaodong,
Long Hao
Publication year - 2013
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12033
Subject(s) - lung , pathology , medicine , metastasis , cancer research , cancer
Background The “seed and soil” hypothesis for metastasis was a pivotal milestone in the study of malignant disease. Recently, growing studies have focused on the tumor secretory factors that may mediate preparation of the “metastatic soil.” A suitable environment for the metastatic lesions was created by many inflammatory cytokines in the lung, whichwas verified by in vivo experimental models. In 2005, a pre‐metastatic niche and metastatic niche modelwere suggested by D avid L yden and B ethan P saila, to delineate the interactions between malignant cells and their microenvironment at the metastatic site, whichsoon became the most importanthypothesis. However, the evidence is limited to animal models. More clinical evidence is needed to support this hypothesis. Methods Human lung specimens were taken from different regions within metastatic lung tissue and normal lung tissue. Differently expressed proteins were analyzed by using the two dimensional fluorescent difference gel electrophoresis (2‐ D DIGE ) technology: about 0.5–1 cm ( T issue 1) of lung tissue was taken adjacent to the metastatic tumor; about 1 cm ( T issue 2) of lung tissue was taken far away from the metastatic tumor; and normal lung tissue of the inflammatory pseudotumor ( T issue 3) was taken at least 3 cm away from the pseudotumor. We usedmatrix‐assisted laser desorption/ ionization time of flight mass spectrometry ( MALDI‐TOF / TOFMS ) analysis to identify differently expressed proteins in T 1, T 2, and T 3 samples. Results T 1 samples were different from T 2 samples in the expression of 27 proteins. T 2 samples had different expressions in 24 proteins, compared to T 3 samples.Nine proteins were expressed differently between T 1 and T 3 samples. These proteins are mainly involved inenergy metabolism, protect the tumor cell from immunologic engraftment of metastatic tumor cells, and migration. Some of thesehave been reported to be related to the tumor metastatic niche hypothesis: Type VI collagen, heat shock protein 90, and Fibrinogen. Conclusion The type VI collagen, heat shock protein 90, and Fibrinogen were selected aspotential niche proteins. These findings support the metastatic niche hypothesis and encourage further studies.