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Dissemination of Vibrio cholerae O1 isolated from Odisha, India
Author(s) -
Nayak Ashish Kumar,
Nayak Smruti Ranjan,
Behera Dipti Ranjan,
Pal Bibhuti Bhusan
Publication year - 2021
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12940
Subject(s) - vibrio cholerae , virulence , biology , microbiology and biotechnology , ciprofloxacin , antibiotic resistance , antibiotics , el tor , cholera , multiplex polymerase chain reaction , gentamicin , norfloxacin , gene , polymerase chain reaction , bacteria , genetics
Summary The present study reported the antimicrobial susceptibility trends, virulence genes, and drug resistance genes of Vibrio cholerae O1 strains isolated from outbreaks and epidemics over two and half decades (1995–2019) from Odisha, India. Antimicrobial susceptibility testing was performed by disc diffusion method. Virulence and drug resistance genes were detected by multiplex PCR assays. All V. cholerae O1 strains were sensitive to gentamicin, chloramphenicol, norfloxacin and ciprofloxacin while resistant to one or more antibiotics used. About 90% of the isolates of V. cholerae O1 carried antibiotic drug resistant genes ( SulII , dfrA1 and strB ) and SXT elements and the results correlated with the phenotypic antibiotic data obtained through disc diffusion assay. The tcpA Haitian variant V. cholerae O1 first appeared in 1999, gradually showing its increasing number upto 2019. TcpA El Tor strains only prevailed from 1995 to 2006; whereas the tcpA classical strains of V.cholerae O1 were found in less number from 1995 to 2016. Two multiplex PCR assays confirmed the presence of various toxigenic and virulence genes ( toxR , ompU , ace , rtxC , ctxA , tcpA , rfbO1 and ompW ) in all isolate of V. cholerae O1 strains. The present findings demonstrated the origin and spread of Haitian variants tcpA in V. cholerae O1 strains over two and half decades.