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Pathogenesis of Salmonella Newport to BALB /c mice when internalized into cherry tomatoes
Author(s) -
MancillaBecerra Lilia Mercedes,
OrozcoGarcía Adriana Guadalupe,
ArmasPuente Priscila,
MartínezChávez Liliana,
BarbaLeón Jeannette
Publication year - 2021
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12930
Subject(s) - salmonella , inoculation , biology , pathogenicity island , microbiology and biotechnology , bacteria , pathogenicity , serotype , gastrointestinal tract , horticulture , biochemistry , genetics
Salmonella Newport is a serovar frequently associated with outbreaks caused by consumption of raw tomatoes. This study tested the internalization of S . Newport‐45 into cherry tomatoes and its resulting pathogenicity in vivo . Pathogenicity of S . Newport‐45 was tested in BALB/c mice inoculated orally with either LB grown or cherry tomatoes homogenates internally contaminated with S . Newport‐45. CFU of S . Newport‐45 was recovered from the gastrointestinal tract, liver and spleen of the inoculated animals. Similar loads ( p > 0.05) were recovered from the GI tract of BALB/c mice inoculated with S . Newport‐45 grown in LB or with cherry tomato homogenates internally contaminated. Spread of S . Newport‐45 to the liver of mice increased ( p < 0.05) when they were inoculated with homogenates of cherry tomatoes internally contaminated with S . Newport‐45 stored for 3 days compared with bacteria grown in LB. Salmonella Newport‐45 hilA and rpoS genes were transcribed when the bacteria were inside the cherry tomato. The results obtained in this study show S . Newport‐45 pathogenicity when it is internalized in a raw consumption fruit such as cherry tomato.