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Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands
Author(s) -
Carvalho Isabel,
TejedorJunco María Teresa,
GonzálezMartín Margarita,
Corbera Juan Alberto,
SuárezPérez Alejandro,
Silva Vanessa,
Igrejas Gilberto,
Torres Carmen,
Poeta Patrícia
Publication year - 2020
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12873
Subject(s) - biology , multilocus sequence typing , escherichia coli , microbiology and biotechnology , phylogenetic tree , cefotaxime , genotype , genetics , gene , antibiotics
Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae , and particularly Escherichia coli , have spread worldwide as one of the most common multidrug‐resistant organisms. The aim of this study was to determine the carriage rate of ESBL‐producing E . coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free‐ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml −1 ). Colonies with typical morphology of E . coli were identified by MALDI‐TOF‐MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double‐disk tests. The presence of bla CTX‐M , bla SHV , bla TEM , bla KPC and bla OXA‐48 genes, as well as mcr‐ 1 (colistin resistance), tet A/ tet B and int 1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL‐producing E . coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried bla CTX‐M genes: bla CTX‐M‐15 ( n = 3) and bla CTX‐M‐55 ( n = 2). ESBL‐positive isolates were ascribed to phylogenetic group D (two isolates), B 1 (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic‐group/ESBL): ST515/B1/CTX‐M‐15, ST1290/A/CTX‐M‐15, ST38/D/CTX‐M‐15, ST457/D/CTX‐M‐55 and ST6448/B 1 /CTX‐M‐55; this suggests a genetic diversity among these isolates. Three CTX‐M‐15‐producing isolates contained the bla TEM gene and one the tet A gene. To our knowledge, this appears to be the first report of ESBL‐producing E . coli in vulture chicks from the Canary Islands.