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Tools to map target genes of bacterial two‐component system response regulators
Author(s) -
Rajeev Lara,
Garber Megan E.,
Mukhopadhyay Aindrila
Publication year - 2020
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12838
Subject(s) - signalling , biology , component (thermodynamics) , computational biology , signalling pathways , genome , gene , synthetic biology , systems biology , bacterial genome size , two component regulatory system , genetics , microbiology and biotechnology , physics , mutant , thermodynamics
Summary Studies on bacterial physiology are incomplete without knowledge of the signalling and regulatory systems that a bacterium uses to sense and respond to its environment. Two‐component systems (TCSs) are among the most prevalent bacterial signalling systems, and they control essential and secondary physiological processes; however, even in model organisms, we lack a complete understanding of the signals sensed, the phosphotransfer partners and the functions regulated by these systems. In this review, we discuss several tools to map the genes targeted by transcriptionally acting TCSs. Many of these tools have been used for studying individual TCSs across diverse species, but systematic approaches to delineate entire signalling networks have been very few. Since genome sequences and high‐throughput technologies are now readily available, the methods presented here can be applied to characterize the entire DNA‐binding TCS signalling network in any bacterial species and are especially useful for non‐model environmental bacteria.