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Distinguishing nutrient‐dependent plant driven bacterial colonization patterns in alfalfa
Author(s) -
Moccia Katherine,
Willems Andrew,
Papoulis Spiridon,
Flores Alicia,
Forister Matthew L.,
Fordyce James A.,
Lebeis Sarah L.
Publication year - 2020
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12815
Subject(s) - colonization , nutrient , biology , medicago sativa , host (biology) , microbiome , inoculation , community structure , botany , ecology , horticulture , bioinformatics
Summary To understand factors that influence the assembly of microbial communities, we inoculated Medicago sativa with a series of nested bacterial synthetic communities and grew plants in distinct nitrogen concentrations. Two isolates in our eight‐member synthetic community, Williamsia sp. R60 and Pantoea sp. R4, consistently dominate community structure across nitrogen regimes. While Pantoea sp. R4 consistently colonizes plants to a higher degree compared to the other six organisms across each community and each nutrient level, Williamsia sp. R60 exhibits nutrient specific colonization differences. Williamsia sp. R60 is more abundant in plants grown at higher nitrogen concentrations, but exhibits the opposite trend when no plant is present, indicating plant‐driven influence over colonization. Our research discovered unique, repeatable colonization phenotypes for individual microbes during plant microbiome assembly, and identified alterations caused by the host plant, microbes, and available nutrients.