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Potential KPC‐2 carbapenemase reservoir of environmental Aeromonas hydrophila and Aeromonas caviae isolates from the effluent of an urban wastewater treatment plant in Japan
Author(s) -
Sekizuka Tsuyoshi,
Inamine Yuba,
Segawa Takaya,
Hashino Masanori,
Yatsu Koji,
Kuroda Makoto
Publication year - 2019
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12772
Subject(s) - aeromonas , aeromonas caviae , aeromonas hydrophila , biology , microbiology and biotechnology , plasmid , enterobacter , wastewater , escherichia coli , bacteria , gene , genetics , biochemistry , engineering , waste management
Summary Aeromonas hydrophila and Aeromonas caviae adapt to saline water environments and are the most predominant Aeromonas species isolated from estuaries. Here, we isolated antimicrobial‐resistant (AMR) Aeromonas strains ( A. hydrophila GSH8‐2 and A. caviae GSH8M‐1) carrying the carabapenemase bla KPC‐2 gene from a wastewater treatment plant (WWTP) effluent in Tokyo Bay (Japan) and determined their complete genome sequences. GSH8‐2 and GSH8M‐1 were classified as newly assigned sequence types ST558 and ST13, suggesting no supportive evidence of clonal dissemination. The strains appear to have acquired bla KPC‐2 ‐positive IncP‐6‐relative plasmids (pGSH8‐2 and pGSH8M‐1‐2) that share a common backbone with plasmids in Aeromonas sp. ASNIH3 isolated from hospital wastewater in the United States, A. hydrophila WCHAH045096 isolated from sewage in China, other clinical isolates ( Klebsiella , Enterobacter and Escherichia coli ), and wastewater isolates ( Citrobacter , Pseudomonas and other Aeromonas spp.). In addition to bla KPC‐2 , pGSH8M‐1‐2 carries an IS 26 ‐mediated composite transposon including a macrolide resistance gene, mph (A). Although Aeromonas species are opportunistic pathogens, they could serve as potential environmental reservoir bacteria for carbapenemase and AMR genes. AMR monitoring from WWTP effluents will contribute to the detection of ongoing AMR dissemination in the environment and might provide an early warning of potential dissemination in clinical settings and communities.