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Low‐iron conditions induces the hypersensitive reaction and pathogenicity hrp genes expression in Xanthomonas and is involved in modulation of hypersensitive response and virulence
Author(s) -
Pandey Sheo Shankar,
Patnana Pradeep Kumar,
Padhi Yasobanta,
Chatterjee Subhadeep
Publication year - 2018
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12650
Subject(s) - xanthomonas campestris , virulence , biology , hypersensitive response , secretion , microbiology and biotechnology , xanthomonas , gene , effector , type three secretion system , xanthomonas oryzae , callose , gene expression , pathogenicity island , pathogen , genetics , biochemistry , plant disease resistance
Summary Expression of hrp ( h ypersensitive r eaction and p athogenicity) genes inside the host is crucial for virulence of phytopathogenic bacteria. The hrp genes encode components of type3 secretion system (T3SS), HR elicitors and several regulators, which are involved in the co‐ordinated expression of hrp genes in the host environment and in hrp inducing chemically defined medium. However, little is known about specific host or environmental factors which may play a role in the induction of hrp gene expression. In this study, we show that iron‐limiting condition elicits induced expression of hrp genes, including type3 secretion system (T3SS) and effectors (T3E). Expression analysis using qRT‐PCR and promoter probe strains suggest significant induction in the expression of Hrp and T3S‐associated genes of Xanthomonas campestris pv. campestris (Xcc) under low‐iron condition, and is suppressed by exogenous supplementation of iron. Furthermore, we show that with exogenous iron supplementation, wild type Xcc exhibited reduced disease symptoms in host‐plant, and exhibited significant reduction in HR and callose deposition in the non‐host plants. Xanthomonas oryzae and oryzicola pathovars also exhibited the iron affect, albeit to a lesser extend compared with the Xcc. Overall, our results suggest that low‐iron condition inside the host may play a crucial role in pathogenicity .