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Interspecies cross‐talk between co‐cultured Pseudomonas putida and Escherichia coli
Author(s) -
MolinaSantiago Carlos,
Udaondo Zulema,
Cordero Baldo F.,
Ramos Juan L.
Publication year - 2017
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12553
Subject(s) - pseudomonas putida , escherichia coli , biology , rhizosphere , efflux , microorganism , pseudomonas , microbiology and biotechnology , pseudomonas aeruginosa , transcriptome , strain (injury) , microbial metabolism , bacteria , gene , biochemistry , gene expression , genetics , anatomy
Summary Pseudomonas putida and Escherichia coli are ubiquitous microorganisms that can be isolated from soil rhizosphere, the surface of vegetables, fresh waters and wastewaters – environments in which they likely co‐exist. Despite this, the potential interactions between these microbes have not been studied in detail. To analyse these interactions, we carried out RNA‐seq transcriptomic analysis of these microbes as monocultures and as co‐cultures. Our results show that co‐culture of these microbes significantly alters transcriptional profiles. The most dramatic transcriptional changes in both microorganisms were involved in central carbon metabolism, as well as adhesion to surfaces and the activation of drug efflux pumps. We also found that acetate production was one of the mechanisms used by E. coli K‐12 MG1655 in response to the presence of P. putida DOT‐T1E.