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Nematopsis temporariae (Gregarinasina, Apicomplexa, Alveolata) is an intracellular infectious agent of tadpole livers
Author(s) -
Chambouvet Aurélie,
Valigurová Andrea,
Pinheiro Lara M.,
Richards Thomas A.,
Jirků Miloslav
Publication year - 2016
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12421
Subject(s) - biology , apicomplexa , zoology , vertebrate , tadpole (physics) , ecology , immunology , genetics , gene , plasmodium falciparum , physics , particle physics , malaria
Summary Amphibians are in decline as a result of habitat destruction, climate change and infectious diseases. Tadpoles are thought susceptible to infections because they are dependent on only an innate immune system (e.g. macrophages). This is because the frog adaptive immune system does not function until later stages of their life cycle. In 1920, Nöller described a putative infectious agent of tadpoles named Nematopsis temporariae , which he putatively assigned to gregarine protists (Apicomplexa). Here, we identify a gregarine infection of tadpoles using both microscopy and ribosomal DNA sequencing of three different frog species ( Rana temporaria , R. dalmatina , and Hyla arborea ). We show that this protist lineage belongs to the subclass Gregarinasina Dufour 1828 and is regularly present in macrophages located in liver sinusoids of tadpoles, confirming the only known case of a gregarine infection of a vertebrate.