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Mucosa‐attached bacterial community in Crohn's disease coheres with the clinical disease activity index
Author(s) -
Schäffler Holger,
Herlemann Daniel P. R.,
Alberts Christian,
Kaschitzki Annika,
Bodammer Peggy,
Bannert Karen,
Köller Thomas,
Warnke Philipp,
Kreikemeyer Bernd,
Lamprecht Georg
Publication year - 2016
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12411
Subject(s) - bacteroidetes , bacteroides , disease , microbiome , crohn's disease , 16s ribosomal rna , nod2 , biology , inflammatory bowel disease , metagenomics , microbiology and biotechnology , dysbiosis , immunology , medicine , bacteria , gene , bioinformatics , biochemistry , genetics
Summary In inflammatory bowel diseases (IBD), microbial communities often become imbalanced suggesting abnormal microbial‐gut interactions. In this study, we analysed the mucosa‐attached gut microbiota from 26 Crohn's disease (CD) patients using 16S rRNA gene amplicon sequencing. The samples were stratified according to their disease activity (Crohn's disease activity index, CDAI). The different disease activity categories had a comparable bacterial richness. Bacterial communities of patients in remission and intermediate CDAI (0–220) were relatively similar and dominated by the genus Bacteroides (>40%). The bacterial composition of patients assigned to a high CDAI category was dominated by Pelomonas (25%) and Flavobacterium (13%) but had a low relative abundance of Bacteroidetes (4%). This indicates the presence of specific abundant bacterial taxa at different CDAI levels. In addition, bacterial communities were also significantly influenced when a tumour necrosis factor (TNF)‐α inhibitor was applied or by the local mucosal inflammation level. As a consequence, a shift of the microbial composition may also indicate a change of the disease activity in CD patients.