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Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA 14
Author(s) -
Fischer Sebastian,
Klockgether Jens,
Morán Losada Patricia,
Chouvarine Philippe,
Cramer Nina,
Davenport Colin F.,
Dethlefsen Sarah,
Dorda Marie,
Goesmann Alexander,
Hilker Rolf,
Mielke Samira,
Schönfelder Torben,
Suerbaum Sebastian,
Türk Oliver,
Woltemate Sabrina,
Wiehlmann Lutz,
Tümmler Burkhard
Publication year - 2016
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12372
Subject(s) - clone (java method) , biology , genome , genetics , pseudomonas aeruginosa , horizontal gene transfer , gene , bacteria
Summary Bacterial populations differentiate at the subspecies level into clonal complexes. Intraclonal genome diversity was studied in 100 isolates of the two dominant P seudomonas aeruginosa clones C and PA 14 collected from the inanimate environment, acute and chronic infections. The core genome was highly conserved among clone members with a median pairwise within‐clone single nucleotide sequence diversity of 8 × 10 −6 for clone C and 2 × 10 −5 for clone PA 14. The composition of the accessory genome was, on the other hand, as variable within the clone as between unrelated clones. Each strain carried a large cargo of unique genes. The two dominant worldwide distributed P . aeruginosa clones combine an almost invariant core with the flexible gain and loss of genetic elements that spread by horizontal transfer.