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The marine bacteria Shewanella frigidimarina NCIMB400 upregulates the type VI secretion system during early biofilm formation
Author(s) -
Linares Denis,
Jean Natacha,
Overtvelt Perrine,
Ouidir Tassadit,
Hardouin Julie,
Blache Yves,
Molmeret Maëlle
Publication year - 2016
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12358
Subject(s) - shewanella oneidensis , biofilm , shewanella , microbiology and biotechnology , secretion , bacteria , biology , secretory protein , type three secretion system , virulence , gene , chemistry , biochemistry , genetics
Summary Shewanella sp. are facultative anaerobic G ram‐negative bacteria, extensively studied for their electron transfer ability. Shewanella frigidimarina has been detected and isolated from marine environments, and in particular, from biofilms. However, its ability to adhere to surfaces and form a biofilm is poorly understood. In this study, we show that the ability to adhere and to form a biofilm of S . frigidimarina NCIMB400 is significantly higher than that of S hewanella oneidensis in our conditions. We also show that this strain forms a biofilm in artificial seawater, whereas in L uria– B ertani, this capacity is reduced. To identify proteins involved in early biofilm formation, a proteomic analysis of sessile versus planktonic membrane‐enriched fractions allowed the identification of several components of the same type VI secretion system gene cluster: putative H cp1 and ImpB proteins as well as a forkhead‐associated domain‐containing protein. The upregulation of H cp1 a marker of active translocation has been confirmed using quantitative reverse transcription polymerase chain reaction. Our data demonstrated the presence of a single and complete type VI secretion system in S . frigidimarina NCIMB400 genome, upregulated in sessile compared with planktonic conditions. The fact that three proteins including the secreted protein H cp1 have been identified may suggest that this type VI secretion system is functional.