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Antimicrobial resistance genes in marine bacteria and human uropathogenic E scherichia coli from a region of intensive aquaculture
Author(s) -
Tomova Alexandra,
Ivanova Larisa,
Buschmann Alejandro H.,
Rioseco Maria Luisa,
Kalsi Rajinder K.,
Godfrey Henry P.,
Cabello Felipe C.
Publication year - 2015
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12327
Subject(s) - bacteria , quinolone , antimicrobial , microbiology and biotechnology , biology , aquaculture , antibiotic resistance , escherichia coli , marine bacteriophage , tetracycline , plasmid , pathogenic bacteria , antibiotics , gene , fishery , genetics , fish <actinopterygii>
Summary Antimicrobials are heavily used in C hilean salmon aquaculture. We previously found significant differences in antimicrobial‐resistant bacteria between sediments from an aquaculture and a non‐aquaculture site. We now show that levels of antimicrobial resistance genes ( ARG ) are significantly higher in antimicrobial‐selected marine bacteria than in unselected bacteria from these sites. While ARG in tetracycline‐ and florfenicol‐selected bacteria from aquaculture and non‐aquaculture sites were equally frequent, there were significantly more plasmid‐mediated quinolone resistance genes per bacterium and significantly higher numbers of qnrB genes in quinolone‐selected bacteria from the aquaculture site. Quinolone‐resistant urinary E scherichia coli from patients in the C hilean aquacultural region were significantly enriched for qnrB (including a novel qnrB gene), qnrS , qnrA and aac(6′)‐1b , compared with isolates from N ew Y ork C ity. Sequences of qnrA1 , qnrB1 and qnrS1 in quinolone‐resistant C hilean E . coli and C hilean marine bacteria were identical, suggesting horizontal gene transfer between antimicrobial‐resistant marine bacteria and human pathogens.