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Interplay between orfamides, sessilins and phenazines in the control of R hizoctonia diseases by P seudomonas sp. CMR 12a
Author(s) -
Olorunleke Feyisara Eyiwumi,
Hua Gia Khuong Hoang,
Kieu Nam Phuong,
Ma Zongwang,
Höfte Monica
Publication year - 2015
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12310
Subject(s) - rhizoctonia solani , mycelium , mutant , biology , phaseolus , rhizoctonia , root rot , brassica , pseudomonas , microbiology and biotechnology , horticulture , biochemistry , bacteria , genetics , gene
Summary We investigated the role of phenazines and cyclic lipopeptides ( CLPs ) (orfamides and sessilins), antagonistic metabolites produced by P seudomonas sp. CMR 12a, in the biological control of damping‐off disease on C hinese cabbage ( B rassica chinensis ) caused by R hizoctonia solani AG 2‐1 and root rot disease on bean ( P haseolus vulgaris L.) caused by R . solani AG 4‐ HGI . A P seudomonas mutant that only produced phenazines suppressed damping‐off disease on C hinese cabbage to the same extent as CMR 12a, while its efficacy to reduce root rot on bean was strongly impaired. In both pathosystems, the phenazine mutant that produced both CLPs was equally effective, but mutants that produced only one CLP lost biocontrol activity. In vitro microscopic assays revealed that mutants that only produced sessilins or orfamides inhibited mycelial growth of R . solani when applied together, while they were ineffective on their own. Phenazine‐1‐carboxamide suppressed mycelial growth of R . solani AG 2‐1 but had no effect on AG 4‐ HGI . Orfamide B suppressed mycelial growth of both R . solani anastomosis groups in a dose‐dependent way. Our results point to an additive interaction between both CLPs . Moreover, phenazines alone are sufficient to suppress Rhizoctonia disease on Chinese cabbage, while they need to work in tandem with the CLPs on bean.