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G eobacter , A naeromyxobacter and A naerolineae populations are enriched on anodes of root exudate‐driven microbial fuel cells in rice field soil
Author(s) -
Cabezas Angela,
Pommerenke Bianca,
Boon Nico,
Friedrich Michael W.
Publication year - 2015
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12277
Subject(s) - microbial population biology , anode , paddy field , biology , terminal restriction fragment length polymorphism , archaea , food science , botany , agronomy , bacteria , chemistry , gene , biochemistry , electrode , restriction fragment length polymorphism , polymerase chain reaction , genetics
Summary Plant‐based sediment microbial fuel cells ( PMFCs ) couple the oxidation of root exudates in living rice plants to current production. We analysed the composition of the microbial community on anodes from PMFC with natural rice field soil as substratum for rice by analysing 16 S rRNA as an indicator of microbial activity and diversity. Terminal restriction fragment length polymorphism (TRFLP) analysis indicated that the active bacterial community on anodes from PMFCs differed strongly compared with controls. Moreover, clones related to D eltaproteobacteria and C hloroflexi were highly abundant (49% and 21%, respectively) on PMFCs anodes. G eobacter (19%), A naeromyxobacter (15%) and A naerolineae (17%) populations were predominant on anodes with natural rice field soil and differed strongly from those previously detected with potting soil. In open circuit ( OC ) control PMFCs , not allowing electron transfer, D eltaproteobacteria (33%), B etaproteobacteria (20%), C hloroflexi (12%), A lphaproteobacteria (10%) and F irmicutes (10%) were detected. The presence of an electron accepting anode also had a strong influence on methanogenic archaea. Hydrogenotrophic methanogens were more active on PMFC (21%) than on OC controls (10%), whereas acetoclastic M ethanosaetaceae were more active on OC controls (31%) compared with PMFCs (9%). In conclusion, electron accepting anodes and rice root exudates selected for distinct potential anode‐reducing microbial populations in rice soil inoculated PMFC .