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Temporal dynamics of active A rchaea in oxygen‐depleted zones of two deep lakes
Author(s) -
Hugoni Mylène,
Domaizon Isabelle,
Taib Najwa,
BiderrePetit Corinne,
Agogué Hélène,
Galand Pierre E.,
Debroas Didier,
Mary Isabelle
Publication year - 2015
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12251
Subject(s) - hypolimnion , metagenomics , ecological niche , biology , ecology , niche , water column , archaea , community structure , pyrosequencing , thaumarchaeota , nutrient , gene , biochemistry , habitat , eutrophication
Summary Deep lakes are of specific interest in the study of archaeal assemblages as chemical stratification in the water column allows niche differentiation and distinct community structure. Active archaeal community and potential nitrifiers were investigated monthly over 1 year by pyrosequencing 16 S rRNA transcripts and genes, and by quantification of archaeal amoA genes in two deep lakes. Our results showed that the active archaeal community patterns of spatial and temporal distribution were different between these lakes. The meromictic lake characterized by a stable redox gradient but variability in nutrient concentrations exhibited large temporal rearrangements of the dominant euryarchaeal phylotypes, suggesting a variety of ecological niches and dynamic archaeal communities in the hypolimnion of this lake. Conversely, T haumarchaeota Marine Group I (MGI) largely dominated in the second lake where deeper water layers exhibited only short periods of complete anoxia and constant low ammonia concentrations. Investigations conducted on archaeal amoA transcripts abundance suggested that not all lacustrine T haumarchaeota conduct the process of nitrification. A high number of 16 S rRNA transcripts associated to crenarchaeal group C 3 or the M iscellaneous E uryarchaeotic G roup indicates the potential for these uncharacterized groups to contribute to nutrient cycling in lakes.