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Culturable endophytic microbial communities in the circumpolar grass, D eschampsia flexuosa in a sub‐ A rctic inland primary succession are habitat and growth stage specific
Author(s) -
Poosakkannu Anbu,
Nissinen Riitta,
Kytöviita MinnaMaarit
Publication year - 2015
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12195
Subject(s) - biology , botany , gammaproteobacteria , ecological succession , internal transcribed spacer , ecology , subarctic climate , ribosomal rna , bacteria , 16s ribosomal rna , gene , biochemistry , genetics
Summary Little is known about endophytic microbes in cold climate plants and how their communities are formed. We compared culturable putative endophytic bacteria and fungi in the ecologically important circumpolar grass, D eschampsia flexuosa growing in two successional stages of subarctic sand dune (68°29′ N ). Sequence analyses of partial 16 S rRNA and internal transcribed spacer (ITS) sequences of culturable endophytes showed that diverse bacteria and fungi inhabit different tissues of D . flexuosa . A total of 178 bacterial isolates representing seven taxonomic divisions, Alpha , Beta and Gammaproteobacteria , A ctinobacteria , B acteroidetes , F irmicutes and A cidobacteria , and 30 fungal isolates representing the phylum A scomycota were identified. Several endophytes were affiliated with specific plant tissues or successional stages. This first report of bacterial endophytes in D . flexuosa revealed that the genus P seudomonas is tightly associated with D . flexuosa , and encompassed 39% of the bacterial isolates, and 58% of seed isolates. Based on 16 S rRNA and ITS sequence data, most of the D . flexuosa endophytes were closely related to microbes from other cold environments. The majority of seed endophytic bacterial isolates were able to solubilize organic form of phosphate suggesting that these endophytes could play a role in resource mobilization in germinating seeds in nutrient‐poor habitat.