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Identification of anaerobic arsenite‐oxidizing and arsenate‐reducing bacteria associated with an alkaline saline lake in K hovsgol, M ongolia
Author(s) -
Hamamura Natsuko,
Itai Takaaki,
Liu Yitai,
Reysenbach AnnaLouise,
Damdinsuren Narantuya,
Inskeep William P.
Publication year - 2014
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12144
Subject(s) - arsenite , arsenate , arsenic , microbiology and biotechnology , 16s ribosomal rna , bacteria , biology , strain (injury) , environmental chemistry , oxidizing agent , chemistry , genetics , organic chemistry , anatomy
Summary Microbial arsenic transformation pathways associated with a saline lake located in northern M ongolia were examined using molecular biological and culturing approaches. Bacterial 16 S rRNA gene sequences recovered from saline lake sediments and soils were affiliated with haloalkaliphiles, including B acillus and H alomonas spp. Diverse sequences of arsenate respiratory reductase ( arrA ) and a new group of arsenite oxidase ( arxA ) genes were also identified. Pure cultures of arsenate‐reducing N itrincola strain and anaerobic arsenite‐oxidizing H alomonas strain were isolated. The chemoorganotrophic H alomonas strain contains arxA gene similar to that of a chemoautotrophic arsenite‐oxidizing A lkalilimnicola ehrlichii strain MLHE ‐1. These results revealed the diversity of arsenic transformation pathways associated with a geographically distinct saline system and the potential contribution of arx ‐dependent arsenite oxidation by heterotrophic bacteria.