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Microbiome diversity of A phis glycines with extensive superinfection in native and invasive populations
Author(s) -
Bansal Raman,
Mian M. A. Rouf,
Michel Andy P.
Publication year - 2014
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12108
Subject(s) - biology , microbiome , abundance (ecology) , soybean aphid , aphid , relative species abundance , superinfection , invasive species , bacteria , metagenomics , ecology , zoology , pest analysis , genetics , gene , botany , aphididae , homoptera , virus
Summary Associations among insects and microbes can lead to beneficial or parasitic interactions. Using 454 sequencing of 16 S RNA genes, we compared microbiome diversity and abundance among field‐collected ( F ) and laboratory‐reared ( L ) populations of the invasive soybean aphid ( A phis glycines ), a pest of soybean. Additionally, we screened A . glycines populations from native ( J apan, S outh K orea and C hina) and invasive regions ( N orth A merica) to broadly determine the microbiome diversity. Our results suggested that A rsenophonus (relative abundance of 54.6%), B uchnera (38.7%) and W olbachia (3.7%) were the major bacteria associated with A . glycines . A rsenophonus was the most abundant in F populations but was significantly reduced in L populations; additional bacteria species also had lower relative abundances in L populations. Native and invasive populations were largely similar in bacteria communities and revealed substantial superinfection of A rsenophonus and W olbachia . The lone exception was a lack of A rsenophonus in A . glycines from J apan. Divergent selection pressures among natural and laboratory populations were inferred as factors driving the differential bacterial communities observed. Our results will allow for improved comparative aphid‐symbiont research and broaden our understanding of the interactions among insects, endosymbionts and their environments.