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Successful enrichment of the ubiquitous freshwater ac I A ctinobacteria
Author(s) -
Garcia Sarahi L.,
McMahon Katherine D.,
Grossart HansPeter,
Warnecke Falk
Publication year - 2014
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12104
Subject(s) - chemistry , food science , business , environmental chemistry
Summary Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for > 50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater A ctinobacteria , diluted freshwater samples from L ake G rosse F uchskuhle, G ermany, were incubated in 96‐well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acI A ctinobacteria of the enrichment belonged to the acI ‐ B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI ‐ B2 was confirmed.