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Genomic analysis of the biocontrol strain P seudomonas fluorescens Pf 29 Arp with evidence of T 3 SS and T 6 SS gene expression on plant roots
Author(s) -
Marchi Muriel,
Boutin Morgane,
Gazengel Kévin,
Rispe Claude,
Gauthier JeanPierre,
GuillermErckelboudt AnneYvonne,
Lebreton Lionel,
Barret Matthieu,
Daval Stéphanie,
Sarniguet Alain
Publication year - 2013
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12048
Subject(s) - biology , pseudomonas fluorescens , microbiology and biotechnology , gene , genome , strain (injury) , gene cluster , bacteria , genetics , anatomy
Summary Several bacterial strains of the P seudomonas genus provide plant growth stimulation, plant protection against pests or bioremediation. Among these bacteria, P . fluorescens Pf 29 Arp reduces the severity of take‐all, a disease caused by the pathogenic fungus G aeumannomyces graminis var. tritici ( Ggt ) on wheat roots. In this study, we obtained a draft genome of Pf 29 Arp and subsequent comparative genomic analyses have revealed that this bacterial strain is closely related to strains of the ‘ P . brassicacearum ‐like’ subgroup including P . brassicacearum ssp. brassicacearum NFM 421 and P . fluorescens F 113. Despite an overall chromosomal organization similar to these strains, a number of features including antibiotic synthesis gene clusters from secondary metabolism are not found in the Pf 29 Arp genome. But Pf 29 Arp possesses different protein secretion systems including type III ( T 3 SS ) and type VI ( T 6 SS ) secretion systems. Pf 29 Arp is the first P seudomonas sp. strain described with four T 6 SS clusters (cluster I , II , III and IV ). In addition, some protein‐coding genes involved in the assembly of these secretion systems are basally expressed during Pf 29 Arp colonization of healthy wheat roots and display different expression patterns on necrotized roots caused by Ggt . These data suggest a role of T 3 SS and T 6 SS in the Pf 29 Arp adaptation to different root environments.