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Distinct lipopeptide production systems for WLIP (white line‐inducing principle) in P seudomonas fluorescens and P seudomonas putida
Author(s) -
RokniZadeh Hassan,
Li Wen,
Yilma Eshetu,
SanchezRodriguez Aminael,
De Mot René
Publication year - 2013
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/1758-2229.12015
Subject(s) - lipopeptide , pseudomonas fluorescens , pseudomonas putida , rhizosphere , biology , microbiology and biotechnology , strain (injury) , pseudomonas , bacteria , genetics , anatomy
Summary The interaction of WLIP (white line‐inducing principle), a member of the viscosin group of P seudomonas lipopeptides, with tolaasin, a lipopeptide mycotoxin secreted by P seudomonas tolaasii , enables identification of the mushroom pathogen relying on formation of a lipopeptide coprecipitate between confronted colonies of an indicator strain (designated P seudomonas ‘reactans’ ) and P . tolaasii . The WLIP non‐ribosomal lipopeptide synthesis system of the mushroom isolate P . ‘reactans’ LMG 5329 ( W ip) was identified and shown to be most similar to the P seudomonas fluorescens SBW 25 viscosin system ( V isc), but remarkably different from the WLIP ‐generating W lp system previously identified in the rice rhizosphere isolate P seudomonas putida RW 10 S 2. The W lp machinery is composed of modules most similar to those recruited for biosynthesis of the non‐viscosin‐type lipopeptides putisolvin and entolysin by strains from the P . putida clade. In line with the pronounced synteny between the wip and visc flanking regions, strain LMG 5329 was identified as an authentic P . fluorescens closely related to strain SBW 25. In both P . putida and P . fluorescens , WLIP production confers similar phenotypes of microbial antagonism and surface colonization. Genotypes other than wlp or wip were not identified among WLIP producers isolated from mushroom, maize rhizosphere or water.