Premium
MiR‐221‐5p is involved in the regulation of inflammatory responses in acute gouty arthritis by targeting IL‐1β
Author(s) -
Li Guangwen,
Zhang Huihui,
Ma Hong,
Qu Shiping,
Xing Qian,
Wang Ge
Publication year - 2021
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/1756-185x.14028
Subject(s) - pathogenesis , proinflammatory cytokine , medicine , microrna , luciferase , arthritis , gout , tumor necrosis factor alpha , inflammation , interleukin , gouty arthritis , untranslated region , real time polymerase chain reaction , microbiology and biotechnology , transfection , messenger rna , cytokine , gene , biology , biochemistry
Aim Gout is caused by the accumulation of deposited monosodium urate (MSU) crystals in the joints. Recent studies have shown that interleukin‐1β (IL‐1β) is a key inflammatory mediator of acute gouty arthritis (AGA), and its level is regulated by microRNAs (miRNAs). The purpose of this study was to study the role of miR‐221‐5p in the pathogenesis of AGA. Methods One hundred patients with AGA and 94 healthy individuals were recruited. The expression of serum miR‐221‐5p was determined by quantitative real‐time polymerase chain reaction. The receiver operating curve (ROC) was applied for diagnostic value analysis. A luciferase reporter assay was performed to confirm the interaction of miRNA and the 3′‐untranslated region (UTR) of IL‐1β. Enzyme‐linked immunosorbent assay was used to detect serum and proinflammatory factors. Results miR‐221‐5p had lower expression in the serum of AGA patients. The area under the curve was 0.884, the sensitivity was 82.0%, and the specificity was 80.9%. Serum miR‐221‐5p was negatively correlated with the expression levels of visual analog scale and IL‐1β. Cell experiments showed that overexpression of miR‐221‐5p significantly inhibited the expression of inflammatory factors tumor necrosis factor‐α, IL‐8, and IL‐1β, while down‐regulation of miR‐221‐5p was the opposite. Luciferase analysis showed that IL‐1β was the target gene of miR‐221‐5p. Conclusions This study confirmed that miR‐221‐5p regulates the production of inflammatory cytokines during the pathogenesis of AGA. These results suggested that miR‐221‐5p could be used as a potential therapeutic target for the treatment of AGA.