Premium
Study on association of human leukocyte antigen‐ DRB 1 alleles amongst Bangladeshi patients with rheumatoid arthritis
Author(s) -
Begum Maksuma,
Sattar Humayun,
Haq Syed A.,
Islam Md. Nazrul,
Saha Sushanta K.,
Hassan M. Masudul,
Saha Ritu,
Kabir Md. Shahriar
Publication year - 2018
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/1756-185x.13291
Subject(s) - medicine , rheumatoid arthritis , immunology , human leukocyte antigen , autoantibody , antigen , allele , population , rheumatoid factor , rheumatology , antibody , gene , genetics , environmental health , biology
Rheumatoid arthritis ( RA ) is a chronic, systemic and autoimmune disease affecting 0.5–1% of the world population. Genetic and environmental factors are already established as being involved in the development of RA . Different human leukocyte antigen ( HLA )‐ DRB 1 alleles have major pathogenic effects to the development of RA . Objective To determine the HLA ‐ DRB 1 allelic frequency of RA in one Bangladeshi tertiary care center. Methods This case–control study was conducted at the Microbiology and Rheumatology Department of Bangabandhu Sheikh Mujib Medical University ( BSMMU ). Fifty‐two patients diagnosed as having RA and 52 healthy controls were enrolled. Buccal swabs were collected from all subjects and HLA ‐ DRB 1 typing was carried out with polymerase chain reaction with sequence‐specific primers ( PCR ‐ SSP ) of low resolution. Blood was also collected for auto‐antibodies (rheumatoid factor [ RF ] and anti‐cyclic citrullinated peptide [anti‐ CCP ]) detection from all subjects. RF was detected by nephelometry and anti‐ CCP was detected by using the enzyme‐linked immunosorbent assay method. Statistical associations of HLA antigen between the groups were determined by chi‐square test. Results In RA patients DR *04 and DR *10 were found at the DRB 1 locus at higher frequencies (20.5%, P = 0.0035 and 18.3%, P = 0.0045, respectively). However, the frequency of DR *15 was significantly lower ( P = 0.005) in RA cases (18.3%) than the control group (35.6%). The frequencies of autoantibodies (anti‐ CCP and RF ) were compared between approximate shared epitope ( SE ) positive and SE negative patients, and no significant association was found. Conclusions In this study DRB 1*04 and DRB 1*10 alleles were significantly associated with RA patients while DRB 1*15 was found more in the control group.