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Serum proteomic profiling in granumomatosis with polyangiitis using two‐dimensional gel electrophoresis along with matrix assisted laser desorption ionization time of flight mass spectrometry
Author(s) -
Rani Lekha,
Minz Ranjana W.,
Arora Amit,
Kannan Monica,
Sharma Aman,
Anand Shashi,
Gupta Dheeraj,
Panda Naresh K.,
Sakhuja Vinay K.
Publication year - 2014
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/1756-185x.12481
Subject(s) - acute phase protein , proteomics , blood proteins , mass spectrometry , two dimensional gel electrophoresis , medicine , microbiology and biotechnology , chemistry , biology , biochemistry , gene , inflammation , chromatography
Abstract Aim The present study is a proteomic approach to find differentially expressed proteins in sera of limited and systemic subsets of active disease versus their remitting state in patients with granulomatosis with polyangiitis ( GPA ) and their correlation with disease activity. Methods Eighteen patients with GPA in active as well as in remitting state and four healthy controls ( HC ) were included in the study. For proteomics analysis, two‐dimensional gel electrophoresis along with matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry were performed. A total of 14 gels were run from pooled patients’ sera from active GPA and remission as well as pooled HC serum. Results There was significant differential expression of proteins in limited versus systemic GPA and between active systemic versus remitting patients of systemic disease. We identified nine maximally differentially expressed and five proteins which were not detected in HC . Among nine proteins, one ( P rolow density lipoprotein receptor‐related protein 1) was downregulated and four proteins (haptoglobin H p, H p2, vitamin D binding protein, killer cell lectin‐like receptor subfamily F member 2), were up‐regulated in both limited and systemic active disease, two proteins like I g gamma‐4 chain C region protein and serum albumin were up‐regulated in limited active GPA and two proteins, that is, cysteine rich secretory protein LCCL domain‐containing 2 precursor and serine‐threonine‐protein kinase A ‐ R af were up‐regulated in systemic active disease. Levels of interleukin‐17 and vitamin‐ D binding protein ( VDBP ) by enzyme‐linked immunosorbent assay could distinctly demarcate active disease versus remission. Conclusion Our study provides potential protein markers of active disease versus remission in GPA .