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Osteoblastic potential of infrapatellar fat pad‐derived mesenchymal stem cells from rheumatoid arthritis and osteoarthritis patients
Author(s) -
Skalska Urszula,
ProchorecSobieszek Monika,
Kontny Ewa
Publication year - 2016
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/1756-185x.12368
Subject(s) - osteopontin , runx2 , infrapatellar fat pad , mesenchymal stem cell , osteoprotegerin , tumor necrosis factor alpha , medicine , adipose tissue , osteoarthritis , endocrinology , cancer research , osteoblast , in vitro , chemistry , microbiology and biotechnology , pathology , biology , receptor , biochemistry , alternative medicine , activator (genetics)
Aim To evaluate the osteoblastic potential of adipose‐derived mesenchymal stem cells ( ASC s) from infrapatellar fat pad ( IPFP ) of rheumatoid arthritis ( RA ) patients in comparison to osteoarthritis ( OA ) patients, as well as the influence of tumor necrosis factor alpha ( TNF α) on osteoblastic ASC differentiation in vitro . Methods ASC s were isolated from IPFP of RA and OA patients. After expansion, cells were cultured in osteogenic medium with or without TNF α. After 2 weeks, expression of BMP‐2 , Runx‐2 , osterix ( Osx ), collagen 1a1 ( Col1a1 ) and osteopontin ( OPN ) messenger RNA (m RNA ) was assessed by reverse transcription polymerase chain reaction and calcium deposition by alizarin red staining. Dickkopf‐1 ( DKK ‐1) and osteoprotegerin ( OPG ) protein concentrations were measured in culture supernatants using enzyme‐linked immunosorbent assay. Results Both RA ‐ and OA ‐ ASC s cultured in osteogenic medium showed calcium deposition. The expression of Runx2 and OPN m RNA was increased in RA ‐ ASC s. These cells expressed significantly more Osx and OPN than OA‐ ASC s. TNFα potentiated calcium deposition, up‐regulated Runx2 and BMP‐2 but down‐regulated Col1a1 and OPN expression. In osteogenic cultures DKK‐1 concentration was increased but that of OPG decreased, whereas TNF α elevated secretion of both cytokines. Conclusion RA ‐ ASC s have comparable or slightly stronger osteogenic potential than OA ‐ ASC s. RA ‐ ASC s seem to be more sensitive to TNF α treatment. TNFα exerts complex effects on ASC osteoblastogenesis, enhances expression of early osteogenic markers and calcium deposition, inhibits expression of m RNA coding for non‐mineral bone components and alters ASC secretory activity.