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Comparison of anti‐mutated citrullinated vimentin, anti‐cyclic citrullinated peptides, anti‐glucose‐6‐phosphate isomerase and anti‐keratin antibodies and rheumatoid factor in the diagnosis of rheumatoid arthritis in Chinese patients
Author(s) -
Zhu Tao,
Feng Liyun
Publication year - 2013
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/1756-185x.12040
Subject(s) - medicine , rheumatoid arthritis , rheumatoid factor , antibody , autoantibody , anti nuclear antibody , immunology , gastroenterology
Aim To evaluate the diagnostic value of anti‐mutated citrullinated vimentin antibodies (anti‐ MCV ), anti‐cyclic citrullinated peptides antibodies (anti‐ CCP ), anti‐glucose‐6‐phosphate isomerase antibodies (anti‐ GPI ) and anti‐keratin antibodies ( AKA ) and rheumatoid factor ( RF ) in rheumatoid arthritis ( RA ). Methods The five auto‐antibodies were detected in serum samples of 56 patients with RA , 21 patients with systemic lupus erythematosus ( SLE ), 11 with ankylosing spondylitis ( AS ), six with Sjögren's syndrome ( SS ), four with connective tissue disease ( CTD ) and 20 healthy controls. Anti‐ MCV , anti‐ CCP and anti‐ GPI were detected by enzyme‐linked immunosorbent assays ( ELISA ), AKA was determined by indirect immunofluorescence and RF was determined by rate nephelometry. Results In RA , anti‐ MCV and anti‐ GPI had the highest sensitivity (78.6% and 75.0%, respectively), anti‐ CCP and AKA had the highest specificity (97.6%). Anti‐ GPI had the lowest specificity (64.3%), and AKA had the lowest sensitivity (48.2%). When two antibodies were detected together, the sensitivity of anti‐ MCV /anti‐ CCP / RF were highest (92.9%) with a lower specificity (73.8%). The combination of anti‐ MCV /anti‐ CCP had a slightly decreased sensitivity (89.3%) and the same specificity (73.8%). Conclusions The combination RF /anti‐ MCV /anti‐ CCP or anti‐ MCV /anti‐ CCP are usefully serologic tests for the diagnosis of RA in Chinese patients.