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Biodiversity and Screening of Moderately Halophilic Bacteria with Hydrolytic and Antimicrobial Activities from Yuncheng Salt Lake, China
Author(s) -
LI Xin,
YU Huiying
Publication year - 2014
Publication title -
acta geologica sinica ‐ english edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 61
eISSN - 1755-6724
pISSN - 1000-9515
DOI - 10.1111/1755-6724.12266_17
Subject(s) - salt lake , halophile , china , citation , library science , beijing , history , biology , computer science , archaeology , bacteria , paleontology , genetics , structural basin
A total of 152 halophilic bacteria were isolated from Yuncheng Salt Lake, China. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that thirty-four strains were related to the phylum Firmicutes and belonged to three families, Bacillaceae, Clostridiaceae and Staphylococcaceae. The other strains were identified as the members of Halomonadaceae and Idiomarinaceae, which belonged to the phylum γ-Proteobacteria. Nine strains showed <97% similarity of 16S rRNA gene sequence compared to other published species, which might represent novel species. The halophilic isolates exhibited various hydrolytic activities. A total of 74, 15, 70, 18, 23 and 3 strains were found to produce extracellular amylase, protease, lipase, cellulase, pectinase and DNAase, respectively. Most hydrolase-producers were members of the genus Halomonas. Combined hydrolytic activities were shown by some strains. Screening of antimicrobial activity indicated that 3, 6, 15, 12, 15 and 16 of halophilic isolates could inhibit Staphylococcus aureus, Escherichia coli, Candida albicans, Fusarium moniliforme, Fusarium semitectum and Fusarium oxysporum, respectively. Results from the present study indicated that halophilic bacteria may be developed as promising sources for novel biocatalysts or bioactive substances in the field of biotechnology.