Open AccessThe effect of allogeneic cardiac stem cells in left ventricular geometry and function in a rat model of myocardial infarction
Author(s) -
Weng Yinlun,
Zhang Xueyuan,
Liu Anmin,
Cai Wangqing,
Li Fangcheng,
Wang Tong
Publication year - 2018
Publication title -
cardiovascular therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 46
eISSN - 1755-5922
pISSN - 1755-5914
DOI - 10.1111/1755-5922.12313
Subject(s) - medicine , ejection fraction , cardiology , myocardial infarction , interventricular septum , diastole , cardiac function curve , ventricular remodeling , ligation , heart failure , preload , artery , blood pressure , hemodynamics , ventricle
Summary Background The development of heart failure after acute myocardial infarction ( MI ) was related to left ventricular ( LV ) pathological remodeling and dysfunction. Cardiac stem cells ( CSC s) provided a new option to treat acute MI . This study was to investigate the effects of CSC s on structural and functional alteration in acute MI . Methods Acute MI was induced in 20 Sprague‐Dawley rats by ligation of the left anterior descending coronary artery. Two weeks after MI , animals were randomized into CSC s or control group. LV geometry and function were echocardiographically measured at baseline, 2, 4, and 6 weeks after MI . After measuring hemodynamics at 6 weeks after MI , hearts were harvested for tracing CSC s stained by PKH 26 and testing expression of VEGF ‐ α / TGF ‐ β 1 by RT ‐ PCR and ELISA . Results Two weeks after MI , there were significant decreases in interventricular septal systolic and diastolic thickness ( IVST s/d), while increases in LV systolic and diastolic dimension ( LVD s/d). Consequently, this contributed to decreases in ejection fraction ( EF ) and fractional shorting ( FS ). With the treatment of CSC s for 4 weeks, significant better ejection fraction ( EF ) and fractional shorting ( FS ) were achieved in CSC s group accompanied by the reduction in LV systole and diastole dimension ( LVD s/d). Besides, a significant improvement in the maximal rate of LV pressure development and decline (peak + d P /d t and ‐ d P /d t , respectively) was observed. Moreover, significantly higher VEGF ‐ α was expressed in CSC s group rather than TGF ‐ β 1 . Conclusion CSC s significantly prevented structural and functional deterioration after MI with increasing expression of VEGF ‐ α.