Open AccessInhibitors of soluble epoxide hydrolase minimize ischemia‐reperfusion‐induced cardiac damage in normal, hypertensive, and diabetic rats
Author(s) -
Islam Oliul,
Patil Prashanth,
Goswami Sumanta K.,
Razdan Rema,
Inamdar Mohammed N.,
Rizwan Mohammed,
Mathew Jubin,
Inceoglu Bora,
Stephen Lee Kin S.,
Hwang Sung H.,
Hammock Bruce D.
Publication year - 2017
Publication title -
cardiovascular therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 46
eISSN - 1755-5922
pISSN - 1755-5914
DOI - 10.1111/1755-5922.12259
Subject(s) - epoxide hydrolase 2 , medicine , ischemia , diabetes mellitus , phenylephrine , aorta , pharmacology , endocrinology , blood pressure , enzyme , biochemistry , chemistry
Summary Aim We designed a study to evaluate the cardioprotective effect of two soluble epoxide hydrolase ( sEH ) inhibitors, 1‐(1‐propanoylpiperidin‐4‐yl)‐3‐(4‐trifluoromethoxy)phenyl)urea ( TPPU ) and trans ‐4‐{4‐[3‐(4‐trifluoromethoxyphenyl)‐ureido]cyclohexyloxy}benzoic acid ( t ‐ TUCB ), in ischemia‐reperfusion ( IR ) model. Methods Cardioprotective effects of the sEH inhibitors were evaluated against IR ‐induced myocardial damage in hearts from normal, hypertensive, and diabetic rats using Langendorff's apparatus. In addition, the effect of sEH inhibitors on endothelial function was evaluated in vitro and ex vivo using isolated rat thoracic aorta. Results Ischemia‐reperfusion ( IR ) increased the myocardial damage in hearts from normal rats. IR ‐induced myocardial damage was augmented in hearts isolated from hypertensive and diabetic rats. Myocardial damage as evident from increase in the activities of lactate dehydrogenase ( LDH ) and creatine kinase‐ MB ( CK ‐ MB ) in heart perfusate was associated with significant decrease in the heart rate and developed tension, and increase in the resting tension in isolated heart. Both sEH inhibitors protected the heart in normal, hypertensive, and diabetic rats subjected to IR injury. The sEH inhibitor t ‐ TUCB relaxed phenylephrine precontracted aorta from normal rats. Relaxant effect of acetylcholine ( AC h) was reduced in aortas from diabetic and hypertensive rats compared to normal rats. Pretreatment of sEH inhibitors to diabetic and hypertensive rats increased relaxant effect of AC h on aortas isolated from these rats. Conclusions Prophylactic treatment with sEH inhibitors decreased myocardial damage due to IR , hypertension and diabetes, and decreased endothelial dysfunction created by diabetes and hypertension. Therefore, inhibitors of sEH are useful probes to study cardiovascular pathology, and inhibition of the sEH is a potential approach in the management of IR ‐induced cardiac damage and endothelial dysfunction‐related cardiovascular disorders.