Open AccessAssociations of P2Y12R gene polymorphisms with susceptibility to coronary heart disease and clinical efficacy of antiplatelet treatment with clopidogrel
Author(s) -
Yang HongHui,
Chen Yan,
Gao ChuanYu
Publication year - 2016
Publication title -
cardiovascular therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 46
eISSN - 1755-5922
pISSN - 1755-5914
DOI - 10.1111/1755-5922.12223
Subject(s) - clopidogrel , medicine , p2y12 , haplotype , genotype , platelet , genotyping , gastroenterology , pharmacology , aspirin , gene , genetics , biology
Summary Objective To investigate the correlations of three P2Y12 receptor (P2Y12R) gene polymorphisms (rs7428575 T>G, rs2046934 C>T, and rs3732759 A>G) with susceptibility to coronary artery disease ( CHD ) and clinical efficacy of clopidogrel treatment for CHD . Methods From May 2014 to May 2015, 178 CHD patients (the case group) and 182 healthy controls (the control group) were selected from our hospital. The platelet‐rich plasma ( PRP ) turbidimetry was used to measure the rate of adenosine diphosphate ( ADP )‐induced platelet aggregation before and after clopidogrel treatment. Clopidogrel‐sensitive group was defined as a 10% or greater decrease in the rate of platelet aggregation after 10 days of clopidogrel treatment, while clopidogrel‐resistant group was defined as a <10% decrease. Genotyping was performed by denaturing high‐performance liquid chromatography ( DHPLC ). A haplotype analysis of P2Y12R gene polymorphisms was performed using SHE sis software. Results There were significant differences in genotype and allele frequencies of rs2046934 C>T and rs3732759 A>G between the case and control groups (all P< .05). Haplotypes GTA and TTA were negatively associated with CHD risk (both P <.05), but haplotype TCA was positively associated with CHD risk ( P =.005). CHD patients in the clopidogrel‐sensitive group had higher frequencies of TT genotype of rs2046934 C>T and lower frequencies of GG genotype of rs3732759 A>G than those in the clopidogrel‐resistant group (both P <.05). Conclusions P2Y12R gene rs2046934 C>T and rs3732759 A>G polymorphisms might be associated with the risk of CHD and the efficacy of clopidogrel treatment for CHD .