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Chromosome‐level genome assembly of the bean bug Riptortus pedestris
Author(s) -
Huang HaiJian,
Ye YuXuan,
Ye ZhuangXin,
Yan XiaoTian,
Wang Xin,
Wei ZhongYan,
Chen JianPing,
Li JunMin,
Sun ZongTao,
Zhang ChuanXi
Publication year - 2021
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.13434
Subject(s) - biology , genome , genetics , gene , chromosome , genome size , bacterial artificial chromosome , zoology
The bean bug ( Riptortus pedestris ) causes great economic losses of soybeans by piercing and sucking pods and seeds. Although R . pedestris has become the focus of numerous studies associated with insect–microbe interactions, plant–insect interactions, and pesticide resistance, a lack of genomic resources has limited deeper insights. Here, we report the first R . pedestris genome at the chromosomal level using PacBio, Illumina, and Hi‐C technologies. The assembled genome was 1.080 Gb in size with a contig N50 of 2.882 Mb. More than 96.3% of the total genome bases were successfully anchored to six unique chromosomes. Genome resequencing of male and female individuals and chromosomic staining demonstrated that the sex chromosome system of R . pedestris is XO, and the shortest chromosome is the X chromosome. In total, 19,026 protein‐coding genes were predicted, 18,745 of which were validated as being expressed. Temporospatial expression of R . pedestris genes in six tissues and 37 development stages revealed 4,657 and 7,793 genes mainly expressed in gonads and egg periods, respectively. Evolutionary analysis demonstrated that R . pedestris and Oncopeltus fasciatus formed a sister group and split ∼80 million years ago (Mya). Additionally, a 5.04 Mb complete genome of symbiotic Serratia marcescens Rip1 was assembled, and the virulence factors that account for successful colonization in the host midgut were identified. The high‐quality R . pedestris genome provides a valuable resource for further research, as well as for the pest management of bug pests.