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NOVOWrap : An automated solution for plastid genome assembly and structure standardization
Author(s) -
Wu Ping,
Xu Chao,
Chen Hao,
Yang Jie,
Zhang Xianchun,
Zhou Shiliang
Publication year - 2021
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.13410
Subject(s) - plastid , genome , biology , genomics , bottleneck , computational biology , genome biology , genetics , computer science , gene , chloroplast , embedded system
Plastid genomes play an important role in genomics and evolutionary biology. Next‐generation sequencing has revolutionized plastid genomic data acquisition to the point that genome assembly has become a bottleneck for widespread utilization of plastid genome data. To solve this problem, we developed an open‐source, cross‐platform tool known as, NOVOWrap , which includes both command‐line and graphical interfaces for automatically assembling plastid genomes on personal computers. With minimal inputs, settings, and user intervention, NOVOWrap can automatically assemble plastid genomes, validate results and standardize the structure using affordable computer resources. The performance of this software has been successfully benchmarked against the plastid genomes of 11 species belonging to lycopods, gymnosperms, and angiosperms. By liberating researchers from laborious and cumbersome computer manipulations and create reliable and standardized genomic data, NOVOWrap is expected to accelerate plastid genome assembly, ease the process of data exchange, and contribute to downstream analysis.