Premium
Long read sequencing of Toona sinensis (A. Juss) Roem: A chromosome‐level reference genome for the family Meliaceae
Author(s) -
Ji YunTao,
Xiu Zhihui,
Chen ChunHai,
Wang Youru,
Yang JingXia,
Sui JuanJuan,
Jiang SanJie,
Wang Ping,
Yue ShaoYun,
Zhang QianQian,
Jin Jiliang,
Wang GuoShu,
Wei QianQian,
Wei Bing,
Wang Juan,
Zhang HaiLin,
Zhang QiuYan,
Liu Jun,
Liu ChangJin,
Jian JianBo,
Qu ChangQing
Publication year - 2021
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.13318
Subject(s) - biology , genome , contig , whole genome sequencing , genome size , sequence assembly , reference genome , genetics , gene , transcriptome , gene expression
Chinese mahogany ( Toona sinensis ) is a woody plant that is widely cultivated in China and Malaysia. Toona sinensis is important economically, including as a nutritious food source, as material for traditional Chinese medicine and as a high‐quality hardwood. However, the absence of a reference genome has hindered in‐depth molecular and evolutionary studies of this plant. In this study, we report a high‐quality T. sinensis genome assembly, with scaffolds anchored to 28 chromosomes and a total assembled length of 596 Mb (contig N50 = 1.5 Mb and scaffold N50 = 21.5 Mb). A total of 34,345 genes were predicted in the genome after homology‐based and de novo annotation analyses. Evolutionary analysis showed that the genomes of T. sinensis and Populus trichocarpa diverged ~99.1–103.1 million years ago, and the T. sinensis genome underwent a recent genome‐wide duplication event at ~7.8 million years and one more ancient whole genome duplication event at ~71.5 million years. These results provide a high‐quality chromosome‐level reference genome for T. sinensis and confirm its evolutionary position at the genomic level. Such information will offer genomic resources to study the molecular mechanism of terpenoid biosynthesis and the formation of flavour compounds, which will further facilitate its molecular breeding. As the first chromosome‐level genome assembled in the family Meliaceae, it will provide unique insights into the evolution of members of the Meliaceae.