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DNA barcodes of the native ray‐finned fishes in Taiwan
Author(s) -
Chang ChiaHao,
Shao KwangTsao,
Lin HanYang,
Chiu YungChieh,
Lee MaoYing,
Liu ShihHui,
Lin PaiLei
Publication year - 2017
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12601
Subject(s) - dna barcoding , biology , barcode , bin , mitochondrial dna , zoology , biodiversity , cytochrome c oxidase subunit i , evolutionary biology , ecology , gene , genetics , algorithm , computer science , operating system
Species identification based on the DNA sequence of a fragment of the cytochrome c oxidase subunit I gene in the mitochondrial genome, DNA barcoding, is widely applied to assist in sustainable exploitation of fish resources and the protection of fish biodiversity. The aim of this study was to establish a reliable barcoding reference database of the native ray‐finned fishes in Taiwan. A total of 2993 individuals, belonging to 1245 species within 637 genera, 184 families and 29 orders of ray‐finned fishes and representing approximately 40% of the recorded ray‐finned fishes in Taiwan, were PCR amplified at the barcode region and bidirectionally sequenced. The mean length of the 2993 barcodes is 549 bp. Mean congeneric K2P distance (15.24%) is approximately 10‐fold higher than the mean conspecific one (1.51%), but approximately 1.4‐fold less than the mean genetic distance between families (20.80%). The Barcode Index Number ( BIN ) discordance report shows that 2993 specimens represent 1275 BIN s and, among them, 86 BIN s are singletons, 570 BIN s are taxonomically concordant, and the other 619 BIN s are taxonomically discordant. Barcode gap analysis also revealed that more than 90% of the collected fishes in this study can be discriminated by DNA barcoding. Overall, the barcoding reference database established by this study reveals the need for taxonomic revisions and voucher specimen rechecks, in addition to assisting in the management of Taiwan's fish resources and diversity.