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Development and preliminary evaluation of a genomewide single nucleotide polymorphisms resource generated by RAD ‐seq for the small yellow croaker ( Larimichthys polyactis )
Author(s) -
Zhang BaiDong,
Xue DongXiu,
Wang Juan,
Li YuLong,
Liu BingJian,
Liu JinXian
Publication year - 2016
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12476
Subject(s) - biology , contig , single nucleotide polymorphism , genetics , population , local adaptation , population genomics , genetic diversity , snp , genomics , nucleotide diversity , computational biology , evolutionary biology , genome , genotype , gene , haplotype , sociology , demography
Recent advances in high‐throughput sequencing technologies have offered the possibility to generate genomewide sequence data to delineate previously unidentified genetic structure, obtain more accurate estimates of demographic parameters and to evaluate potential adaptive divergence. Here, we identified 27 556 single nucleotide polymorphisms for the small yellow croaker ( Larimichthys polyactis ) using restriction‐site‐associated DNA ( RAD ) sequencing of 24 individuals from two populations. Significant sources of genetic variation were identified, with an average nucleotide diversity ( π ) of 0.00105 ± 0.000425 across individuals, and long‐term effective population size was thus estimated to range between 26 172 and 261 716. According to the results, no differentiation between the two populations was detected based on the SNP data set of top quality score per contig or neutral loci. However, the two analysed populations were highly differentiated based on SNP data set of both top F ST value per contig and the outlier SNP s. Moreover, local adaptation was highlighted by an F ST ‐based outlier tests implemented in LOSITAN and a total of 538 potentially locally selected SNP s were identified. blast2go annotation of contigs containing the outlier SNP s yielded hits for 37 (66%) of 56 significant blastx matches. Candidate genes for local adaptation constituted a wide array of biological functions, including cellular response to oxidative stress, actin filament binding, ion transmembrane transport and synapse assembly. The generated SNP resources in this study provided a valuable tool for future population genetics and genomics studies of L. polyactis .