Premium
Development of a N asonia vitripennis outbred laboratory population for genetic analysis
Author(s) -
Zande Louis,
Ferber Steven,
Haan Ammerins,
Beukeboom Leo W.,
Heerwaarden Joost,
Pannebakker Bart A.
Publication year - 2014
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12201
Subject(s) - biology , nasonia vitripennis , population , genetic variation , genetics , evolutionary biology , genome , population genetics , genetic analysis , quantitative trait locus , gene , parasitoid , pteromalidae , host (biology) , demography , sociology
The parasitoid wasp genus N asonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high‐resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding N asonia biology. To further advance the utility of N asonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well‐characterized experimental population. In this study, we describe the establishment of the genetically diverse HVR x laboratory population from strains collected from the field in the N etherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size ( N e ) by microsatellite analysis. A genome‐wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high‐quality SNP s spanning all five N asonia chromosomes. The HVR x population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the N asonia model system.