Premium
Comparative studies on species identification of Noctuoidea moths in two nature reserve conservation zones ( B eijing, C hina) using DNA barcodes and thin‐film biosensor chips
Author(s) -
Yang F.,
Shi Z. Y.,
Bai S. L.,
Ward R. D.,
Zhang A. B.
Publication year - 2014
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12165
Subject(s) - dna barcoding , biology , biosensor , barcode , identification (biology) , pest analysis , polymerase chain reaction , dna , computational biology , microbiology and biotechnology , gene , botany , genetics , evolutionary biology , biochemistry , computer science , operating system
Abstract Rapid and accurate identification of species is required for the biological control of pest N octuoidea moths. DNA barcodes and thin‐film biosensor chips are two molecular approaches that have gained wide attention. Here, we compare these two methods for the identification of a limited number of N octuoidea moth species. Based on the commonly used mitochondrial gene cytochrome c oxidase I (the standard DNA barcode for animal species), 14 probes were designed and synthesized for 14 species shared by two national nature reserves in B eijing and H ebei, C hina. Probes ranged in length from 18 to 27 bp and were designed as mismatch probes to guarantee that there were at least three base differences between the probe and nontarget sequences. The results on the chip could be detected by the naked eye without needing special equipment. No cross‐hybridizations were detected although we tested all probes on the 14 target and 24 nontarget Noctuoidea species. The neighbour‐joining tree of the 38 species based on COI sequences gave 38 highly supported independent groups. Both DNA barcoding and thin‐film biosensor chips, based on the COI gene, are able to accurately identify and discriminate the 14 targeted moth species in this study. Because of its speed, high accuracy and low cost, the thin‐film biosensor chip is a very practical means of species identification. Now, a more comprehensive chip will be developed for the identification of additional Noctuoidea moths for pest control and ecological protection.