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Genetic assessment of safflower ( C arthamus tinctorius L .) collection with microsatellite markers acquired via pyrosequencing method
Author(s) -
Lee GiAn,
Sung JungSook,
Lee SokYoung,
Chung JongWook,
Yi JungYoon,
Kim YeonGyu,
Lee MyungChul
Publication year - 2014
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12146
Subject(s) - biology , genetic diversity , microsatellite , germplasm , genetic structure , loss of heterozygosity , pyrosequencing , genetics , population , genetic marker , allele , genetic variation , evolutionary biology , gene , botany , demography , sociology
A genetic evaluation of safflower germplasm collections derived from different geographical regions and countries will provide useful information for sustainable conservation and the utilization of genetic diversity. However, the molecular marker information is limited for evaluation of genetic diversity of safflower germplasm. In this study, we acquired 509 putative genomic SSR markers for sufficient genome coverage using next‐generation sequencing methods and characterized thirty polymorphic SSR s in safflower collection composed of 100 diverse accessions. The average allele number and expected heterozygosity were 2.8 and 0.386, respectively. Analysis of population structure and phylogeny based on thirty SSR profiles revealed genetic admixture between geographical regions contrary to genetic clustering. However, the accessions from Korea were genetically conserved in distinctive groups in contrast to other safflower gene pool. In conclusion, these new genomic SSR s will facilitate valuable studies to clarify genetic relationships as well as conduct population structure analyses, genetic map construction and association analysis for safflower.