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Advancing nematode barcoding: A primer cocktail for the cytochrome c oxidase subunit I gene from vertebrate parasitic nematodes
Author(s) -
Prosser Sean W. J.,
VelardeAguilar Maria G.,
LeónRègag Virginia,
Hebert Paul D. N.
Publication year - 2013
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12082
Subject(s) - biology , dna barcoding , phylum , cytochrome c oxidase subunit i , primer (cosmetics) , nematode , evolutionary biology , cytochrome c oxidase , phylogenetics , vertebrate , mitochondrial dna , gene , barcode , zoology , genetics , ecology , mitochondrion , chemistry , organic chemistry , computer science , operating system
Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species‐level resolution in all groups, and those that do lack primer sets effective across the phylum, precluding high‐throughput processing. This study describes a cocktail of three novel primer pairs that overcome this limitation by recovering cytochrome c oxidase I ( COI ) barcodes from diverse nematode lineages parasitic on vertebrates, including members of three orders and eight families. Its effectiveness across a broad range of nematodes enables high‐throughput processing.