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Molecular identification of dipteran pests (Diptera: Sciaroidea) from shiitake mushroom
Author(s) -
Shin Seunggwan,
Jung Sunghoon,
Lee Heungsik,
Lee Seunghwan
Publication year - 2013
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12057
Subject(s) - biology , mushroom , maggot , cytochrome c oxidase subunit i , larva , dna barcoding , insect , botany , calliphoridae , pest analysis , zoology , horticulture , mitochondrial dna , gene , genetics
On shiitake farms, mycophagous maggots can cause serious damage by preventing formation of the fruiting body. Recently, these pests have significantly reduced shiitake production in Korea. However, larvae and female adults cannot be identified due to their lack of morphological characteristics. Therefore, farmers and applied entomologists are unable to determine which species is the primary cause of the shiitake damage. In this study, mycophagous flies (colonized larvae) were collected from damaged shiitake farms and subsequently identified by matching identified males with the cytochrome c oxidase subunit I ( COI ) sequences from the larvae. Divergences of the COI sequences among the species discriminated the clusters clearly, and the mycophagous pests were identified as C amptomyia corticalis and C . heterobia . Interestingly, these two species coexisted under the bark of shiitake oak bed logs.