Premium
Primer design for identifying economically important L iriomyza species ( D iptera: A gromyzidae) by multiplex PCR
Author(s) -
Nakamura Shigeo,
Masuda Toshio,
Mochizuki Atsushi,
Konishi Kazuhiko,
Tokumaru Susumu,
Ueno Keiichiro,
Yamaguchi Takuhiro
Publication year - 2013
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/1755-0998.12025
Subject(s) - biology , primer (cosmetics) , mitochondrial dna , botany , microbiology and biotechnology , gene , genetics , chemistry , organic chemistry
Leafminer flies, especially, L iriomyza huidobrensis , L iriomyza sativae and L iriomyza trifolii , are quarantine species in many countries. Their morphological similarity makes identification difficult. To develop a rapid, reliable, sensitive and simple molecular identification method using multiplex PCR , we newly sequenced the mitochondrial cytochrome oxidase I ( COI ) genes of L iriomyza bryoniae , L iriomyza chinensis , L . huidobrensis , L . sativae , L . trifolii , C hromatomyia horticola and four parasitoid species. We aligned them with all the COI sequences of the leafminer flies found in the international DNA nucleotide sequence databases ( DDBJ / EMBL / G en B ank). We then designed species‐specific primers to allow us to differentiate between L . bryoniae , L . chinensis , L . huidobrensis , L . sativae , and L . trifolii .