Open Access中药复方抑制α‐葡萄糖苷酶和α‐淀粉酶治疗2型糖尿病:电子反向对接与体外酶试验的验证
Author(s) -
Tolmie Morné,
Bester Megan Jean,
Apostolides Zeno
Publication year - 2021
Publication title -
journal of diabetes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.949
H-Index - 43
eISSN - 1753-0407
pISSN - 1753-0393
DOI - 10.1111/1753-0407.13163
Subject(s) - acarbose , piperine , rosmarinic acid , apigenin , cinnamic acid , cuminum , postprandial , biochemistry , traditional medicine , amylase , medicine , pharmacology , chemistry , food science , enzyme , flavonoid , diabetes mellitus , antioxidant , essential oil , endocrinology
Background α‐Amylase and α‐glucosidase are important therapeutic targets for the management of type 2 diabetes mellitus. The inhibition of these enzymes decreases postprandial hyperglycemia. In the present study, compounds found in commercially available herbs and spices were tested for their ability to inhibit α‐amylase and α‐glucosidase. These compounds were acetyleugenol, apigenin, cinnamic acid, eriodictyol, myrcene, piperine, and rosmarinic acid. Methods The enzyme inhibitory nature of the compounds was evaluated using in silico docking analysis with Maestro software and was further confirmed by in vitro α‐amylase and α‐glucosidase biochemical assays. Results The relationships between the in silico and in vitro results were well correlated; a more negative docking score was associated with a higher in vitro inhibitory activity. There was no significant ( P > .05) difference between the inhibition constant (K i ) value of acarbose, a widely prescribed α‐glucosidase and α‐amylase inhibitor, and those of apigenin, eriodictyol, and piperine. For α‐amylase, there was no significant ( P > .05) difference between the K i value of acarbose and those of apigenin, cinnamic acid, and rosmarinic acid. The effect of the herbal compounds on cell viability was assessed with the sulforhodamine B (SRB) assay in C2C12 and HepG2 cells. Acetyleugenol, cinnamic acid, myrcene, piperine, and rosmarinic acid had similar ( P > .05) IC 50 values to acarbose. Conclusions Several of the herbal compounds studied could regulate postprandial hyperglycemia. Using herbal plants has several advantages including low cost, natural origin, and easy cultivation. These compounds can easily be consumed as teas or as herbs and spices to flavor food.