Open AccessAldehyde inhibition of antioxidant enzymes in the blood of diabetic patients
Author(s) -
Lankin Vadim Z.,
Konovalova Galina G.,
Tikhaze Alla K.,
Shumaev Konstantin B.,
Belova Kumskova Elena M.,
Grechnikova Maria A.,
Viigimaa Margus
Publication year - 2016
Publication title -
journal of diabetes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.949
H-Index - 43
eISSN - 1753-0407
pISSN - 1753-0393
DOI - 10.1111/1753-0407.12309
Subject(s) - medicine , diabetes mellitus , antioxidant , enzyme , aldehyde dehydrogenase , pharmacology , endocrinology , biochemistry , chemistry
Background The aim of the present study was to examine the effect of aldehyde modification on antioxidant enzyme activity in diabetic patients. Methods The activity of commercially available antioxidant enzymes (catalase, glutathione peroxidase [ GPx ], and C u, Z n‐superoxide dismutase [ SOD ]) was determined in vitro prior to and after aldehyde modification. The activity of erythrocyte C u, Z n‐ SOD was assayed in blood drawn from healthy donors, diabetic patients with decompensated carbohydrate metabolism, and diabetic patients after glucose‐lowering therapy. Results In vitro aldehyde modification had no effect on catalase activity, but diminished GPx and C u, Z n‐ SOD activity. In diabetic patients with decompensated carbohydrate metabolism, glucose‐lowering therapy significantly increased C u, Z n‐ SOD activity, the effect being especially pronounced after administration of metformin. Conclusions It is likely that metformin antagonizes the aldehyde‐induced inhibition of erythrocyte C u, Z n‐ SOD in diabetic patients more effectively than sulfonylurea drugs.