Open AccessEnumeration and functional investigation of endothelial progenitor cells in neovascularization of diabetic foot ulcer rats with a C hinese 2‐herb formula
Author(s) -
Tam Jacqueline Chor Wing,
Ko Chun Hay,
Lau Kit Man,
To Ming Ho,
Kwok Hin Fai,
Siu Wing Sum,
Lau Ching Po,
Chan Wai Yee,
Leung Ping Chung,
Fung Kwok Pui,
Lau Clara Bik San
Publication year - 2015
Publication title -
journal of diabetes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.949
H-Index - 43
eISSN - 1753-0407
pISSN - 1753-0393
DOI - 10.1111/1753-0407.12230
Subject(s) - neovascularization , medicine , angiogenesis , progenitor cell , endothelial progenitor cell , stromal cell , diabetic foot , wound healing , flow cytometry , diabetic foot ulcer , diabetes mellitus , cancer research , endocrinology , immunology , stem cell , biology , microbiology and biotechnology
Backbround We investigated the effect of a C hinese 2‐herb formula ( NF 3) on the enumeration and angiogenic differentiation of endothelial progenitor cells ( EPC s) in diabetic foot ulcer rats. Methods EPC s and stromal cell‐derived factor‐1α ( SDF ‐1α) were quantified by flow cytometry and ELISA , respectively. In vitro angiogenesis assays included proliferation, adhesion, migration and tube formation. Results Our result demonstrated that NF 3 (0.98 g/kg) could significantly enhance the circulating CD 34 + / VEGFR 2 + / CD 45 − EPC s levels in diabetic foot ulcer rats by 60% ( P < 0.05) through the partial elevation of SDF ‐1α, restoring the mobilization ability of EPC s for wound neovascularization. We successfully isolated the BM ‐derived EPC s to study their angiogenic potential after NF 3 treatment. BM ‐derived EPC s significantly expressed cell surface markers of CD 34, CD 146 and VEGFR 2 ( P < 0.05 − 0.01). NF 3 could significantly stimulate the proliferation and attachment ability of EPC s dose‐dependently ( P < 0.01–0.001). Besides, NF 3 could significantly augment EPC s migration ( P < 0.001) and tube formation ( P < 0.01–0.001). Conclusions NF 3 modulated diabetic wound healing through regulation of systemic EPC s level and increase in local vascular formation.