Open AccessInhibition of α‐glucosidase and glucose intestinal absorption by T hymelaea hirsuta fractions (毛花瑞香成分对α‐糖甙酶以及肠道葡萄糖吸收的抑制作用)
Author(s) -
Abid Sanae,
Lekchiri Abdenbi,
Mekhfi Hassane,
Ziyyat Abderrahim,
Legssyer Abdekhaleq,
Aziz Mohamed,
Bnouham Mohamed
Publication year - 2014
Publication title -
journal of diabetes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.949
H-Index - 43
eISSN - 1753-0407
pISSN - 1753-0393
DOI - 10.1111/1753-0407.12106
Subject(s) - postprandial , in vivo , in vitro , medicine , absorption (acoustics) , diabetes mellitus , pharmacology , biochemistry , endocrinology , chemistry , biology , physics , microbiology and biotechnology , acoustics
Background T hymelaea hirsuta ( L .) E ndl. ( T hymelaeaceae) is a medicinal plant used in M orocco to treat diabetes. In previous studies T . hirsuta has shown a potent antihyperglycemic effect. Our aim was to study the effect of the plant on α‐glucosidase inhibition and intestinal glucose absorption. Methods Five fractions of T . hirsuta were tested, in vitro, in vivo and, in situ, to elucidate the inhibition of α‐glucosidase and intestinal glucose uptake. Results The fractions induced, in vitro, a significant inhibition of α‐glucosidase. The ethyl acetate fraction ( EATh ) had high activity and its inhibition mode was non‐competitive. The EATh at 50 and 100 mg/kg doses, decreased significantly, in vivo, the postprandial hyperglycemia after sucrose loading in normal and diabetic mice. Moreover, 50 mg/kg of EATh significantly decreased intestinal glucose uptake, in situ, in rats. Conclusion The antihyperglycemic effect of T . hirsuta can be explained, in part, by the inhibition of intestinal α‐glucosidase and intestinal glucose absorption.