Resveratrol exhibits differential protective effects on fast‐ and slow‐twitch muscles in streptozotocin‐induced diabetic rats (在链脲霉素诱导的糖尿病大鼠中，白藜芦醇对快反应与慢反应肌肉的保护效应具有差别)

Background This study aimed to investigate the differential protective effect of resveratrol ( RSV ) on oxidative stress and metabolic signaling pathways in fast‐ and slow‐twitch skeletal muscles of rats with diabetes. Methods Diabetic rats were induced by streptozotocin ( STZ ) for 2 weeks and then administered with RSV (1, 10 and 100 μg/kg per day) for 1 week. We determined oxidative stress and protein expression by lucigenin‐mediated chemiluminescence and Western immunoblot. Results The superoxide anion production and copper‐zinc superoxide dismutase ( CuZnSOD ) protein level were increased in fast‐twitch muscle than in slow‐twitch muscle of diabetes. The Akt and glycogen synthase kinase 3 ( GSK ‐3) phosphorylations were reduced in both fast‐ and slow‐twitch muscles of diabetes. Oxidative stress and GSK ‐3 dephosphorylation were corrected by RSV treatment in both fast‐ and slow‐twitch muscles of diabetes. Furthermore, RSV treatment downregulated CuZnSOD protein level in diabetic fast‐twitch muscle. In diabetic slow‐twitch muscle, RSV treatment elevated manganese SOD ( MnSOD ) and phosphorylated A kt protein levels and reduced acetyl‐ CoA carboxylase ( ACC ) phosphorylation. Conclusions Our results suggested that fast‐twitch muscle incurred more oxidative stress, whereas slow‐twitch muscle altered metabolic signaling molecules activities under diabetic status. The antidiabetic effect of RSV on fast‐ and slow‐twitch skeletal muscles was mediated by different antioxidative and metabolic signals.